Procedure:

1.  At the beginning of surgery, record time and body temperature using a CyQ^® 111 Small Animal Thermometer

2.  Using operating scissors, make a 3/4" incision over femoral sheath

3.  Remove fascia

4.  Pull back abdominal muscles with 5" curved halsted-mosquito hemostats (Roboz, #RS-7114 or similar)

5.  Using 4" curved blunt micro dissecting forceps (Roboz, #RS-5137), tease away sheath around nerve and artery/vein

6.  Carefully tease away sheath between artery and vein and isolate about 1" of artery

7.  Place ligature (4-0 silk) in distal and proximal positions around isolated artery

8.  Tie off distal ligature and hold taunt with hemostats

9.  Place 1 1/2" Diffenbach serrefine clamp (Roboz #RS-7422), on most proximal portion of exposed artery

10.  Using 3" Vannas micro dissecting scissors (Roboz #RS-5610 or similar), make a small incision half way through the artery close to the distal ligature.  Do not cut all the way through the artery (place a few drops of lidocaine, obtained from your animal resources center, directly on the vessel to relax the vessel)

11.  Insert catheter into opening in artery, and push catheter to clamp
       Catheter:   fill 1cc syringe with 5:1 Ringers:herapin (0.2cc herapin up to 1cc with Ringers) - put 23 gauge needle on syringe (break off tip by first scoring with file) - cut off 6-10" of tubing, cut beveled edge tubing: PE-50; ID 0.023"; OD 0.038" - fill tube with herapin solution

12.  Check for visible pooling of blood within catheter tube

13.  Tie down tube with both ligatures (several double knots)

14.  Cut off ends of ligatures near knots

15.  Hook catheter up to any CyQ^® Blood Pressure Monitor

16.  Record time, temperature, and blood pressure

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